Anthony Joshua will put his four title belts on the line against Oleksandr Usyk at the Tottenham Hotspur Stadium on September 25.
The 31-year-old currently holds the WBA, IBF, WBO and IBO titles.
While he wanted to unify the division against Tyson Fury, he has been ordered to defend his WBO title against mandatory challenger Usyk.
“The date is set and we are fully locked in.
“I put it all on the line again and it’s time to defend my crown.
“We are two Olympic Gold medallists who have fought our way to the top and never avoided challenges,” Joshua, who toured the stadium prior to Tuesday’s announcement, said.
Joshua is the first prizefighter to headline an event at the Tottenham Hotspur Stadium.
Spurs’ stunning new 60,000-seater stadium was due to host Joshua’s fight with Kubrat Pulev last year until the coronavirus crisis forced the bout indoors to Wembley Arena, in front of only 1,000 supporters.
He returns to London for his fifth stadium fight and fans will see Joshua following in the footsteps of former British fighter Frank Bruno, who defeated Joe Bugner in Matchroom Boxing’s first heavyweight promotion at the old White Hart Lane in October 1987.